The EPPIC Program is intended to provide the Rutgers research community with expertise and technical assistance for the study of ion channel function and cellular electrophysiology. We have a long-standing interest in ion channel structure, function, and pharmacology, with previous work focusing on a wide variety of channels, including K2P, calcium-activated, and ATP-sensitive potassium channels, TRP channels, calcium-activated chloride channels, and mechanosensitive ion channels. Through collaborations with colleagues across Rutgers, we contribute to the design and implementation of multiple approaches, including patch clamp electrophysiology, physiology studies in animal models of disease, and establishing a range of biochemical assays to measure electrophysiological behavior. As part of this program, we also seek to build links with external electrophysiology experts and to present the work at Rutgers to the wider community.

Contact us if you are interested in ion channel studies and need a hand!

Within the McClenaghan lab we have two electrophysiology set-ups with fluorescence microscopes allowing for independent or simultaneous patch clamp and fluorescent indicator experiments, two-electrode voltage clamp apparatus for those stubborn to express channels, and rodent ECG and hemodynamic recording equipment. In addition, we have access to plate readers with integrated liquid handling and we are developing methods for high-throughput mutagenesis screening. With these facilities we can make patch clamp and sharp electrode recordings of recombinant channels in heterologous systems or in isolated native cells, as well as conduct a range of fluorescent indicator assays for calcium, potassium, chloride or non-selective channels. Equipment is available for collaborators to use and training in techniques can be offered when needed. We also support collaborators with assay design, generation of preliminary data, and analysis.